Coculture of DCs and cells to be taken up (1(-2)x10^5 + 1(-2)x10^5 cells in 12-well) for 4 h to 24 h at 37°C

Purified CD8 T cells using MACS cell separation are co-cultured with electroporated DC.

Untouched naive CD4+ T cells are co-coltured with fungi -pulsed DCs

The T cell transfer will consist of T cells expanded by co-culture with tumor lysate loaded DC.