Staining of the cells in PEalphaDC80 in PBS + 2 % FCS

Staining of D2SC1/Flt3-DC and staining of the cells to be taken up by Mini67-1KT or Mini26-1KT (PKH67/PKH26 green/red fluorescent cell linker mini kit for general cell membrane labelling) from Sigma

Staining of GM-DC/Raw with anti-CD11c antibodies