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DC pulsing
adding substance to cell culture stepProtocol: cGMP DC generation
On day 5 of culture immature DC will be pulsed with irradiated tumor cells (apoptotic bodies) prepared from the removed lesion and matured for 48 hours in presence of TNF-alpha. Monocyte and DC viability as well as functionality will be continuously monitored.
created over 16 years ago
(2 March 2009)
last modified over 13 years ago
(28 September 2011)
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