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Protocol Steps  >  data transformation step  >  Adjustment of intensity values

Adjustment of intensity values

data transformation step

Protocol: Yeast experiment: Yeast Microarray Protocol

Intensity values are adjusted by subtracting surrounding background from spots. The median of spot intensities is corrected for background. To eliminate signals that are most prone to estimation error, any spot is excluded from analysis if both the Cy3 and Cy5 mean fluorescence signals are within two standard deviations of the mean background signals for that spot. This procedure avoids artificially inflated measurements of expression due to low signals. Additional to eliminating flagged spots, spots are also visually inspected and flawed ones discarded from the analysis.

created over 14 years ago (2 March 2009)    last modified over 12 years ago (28 September 2011)   [ RDF Rdf ]   [ RelFinder Relfinder ]