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Protocol Steps  >  establishing cell culture step  >  Cell culture

Cell culture

establishing cell culture step

Protocol: DCs differentiation and stimulation

Cells are cultured in RPMI 1640 medium (GIBCO BRL) supplemented with 2 mM L-glutamine (Sigma), 1% (vol/vol) non-essential amino acids, 100 mM sodium pyruvate, 50 U/ml of penicillin and 50 mg/ml of streptomycin (Gibco BRL) containing 10% (vol/vol) FCS (Hyclone).

created over 14 years ago (2 March 2009)    last modified over 11 years ago (28 September 2011)   [ RDF Rdf ]   [ RelFinder Relfinder ]