IL-12p70 inhibition by cytochalasin D

Protocol: IL-12p70 blocking assay

DCs are exposed to cytochalasin D (10 microg/ml, TebuBio) for 30 minutes at 4°C. After washing with cold PBS, DCs are stimulated with S. cerevisiae yeast cells or spores at a DC:stimuli ratio of 4:1 for 24 hours. IL-12p70 production is assessed by ELISA.

created over 15 years ago (21 January 2010)    last modified over 13 years ago (28 September 2011)   [ RDF ]   [ RelFinder ]