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Protocol Steps  >  blocking step  >  IL-12p70 inhibition by cyto...

IL-12p70 inhibition by cytochalasin D

blocking step

Protocol: IL-12p70 blocking assay

DCs are exposed to cytochalasin D (10 microg/ml, TebuBio) for 30 minutes at 4°C. After washing with cold PBS, DCs are stimulated with S. cerevisiae yeast cells or spores at a DC:stimuli ratio of 4:1 for 24 hours. IL-12p70 production is assessed by ELISA.

created over 13 years ago (21 January 2010)    last modified over 11 years ago (28 September 2011)   [ RDF Rdf ]   [ RelFinder Relfinder ]