Adaptation of Kurimoto cDNA amplification method
Laboratory protocolWe have adapted the cDNA amplification method of Kurimoto et al. (Nat Protoc 2007, 2:739) to cDNA extracted from small numbers (±100) of cells microdissected from human tumor sections. We are currently checking the accuracy and reproducibility of microarray (Affymetrix) data obtained through this procedure.
Protocol Steps:
created over 15 years ago (11 January 2010) last modified over 13 years ago (28 September 2011)  [ RDF ]  [ RelFinder ]