Adaptation of Kurimoto cDNA amplification method
Laboratory protocolWe have adapted the cDNA amplification method of Kurimoto et al. (Nat Protoc 2007, 2:739) to cDNA extracted from small numbers (±100) of cells microdissected from human tumor sections. We are currently checking the accuracy and reproducibility of microarray (Affymetrix) data obtained through this procedure.
Protocol Steps:

created over 15 years ago
(11 January 2010)
last modified over 14 years ago
(28 September 2011)
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