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Protocols  >  Laboratory protocol  >  Adaptation of Kurimoto cDNA...

Adaptation of Kurimoto cDNA amplification method

Laboratory protocol

We have adapted the cDNA amplification method of Kurimoto et al. (Nat Protoc 2007, 2:739) to cDNA extracted from small numbers (±100) of cells microdissected from human tumor sections. We are currently checking the accuracy and reproducibility of microarray (Affymetrix) data obtained through this procedure.

Protocol Steps:
Adaptation of Kurimoto cDNA amplification method Graph

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