Adaptation of Kurimoto cDNA amplification method
We have adapted the cDNA amplification method of Kurimoto et al. (Nat Protoc 2007, 2:739) to cDNA extracted from small numbers (±100) of cells microdissected from human tumor sections. We are currently checking the accuracy and reproducibility of microarray (Affymetrix) data obtained through this procedure.
protocol
Laboratory protocol
Global single-cell cDNA amplification to provide a template for representative high-density oligonucleotide microarray analysis.
Pierre Coulie