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Lentiviral infection of target cells

Laboratory protocol

Lentiviral infection of target cells:
Day 1: seed cells
Day 2: remove the medium from the cells. Mix the medium containing the virus gently by pipetting and add to the cells (+ 6 ?g/ml of polybrene). Incubate the cells at 37°C overnight.
Day 3: change medium and start selection for stably transduced cells

Protocol Steps:
  • Cell seeding Day 1: seed cells
  • Incubation of cells Day 2: remove the medium from the cells. Mix the medium containing virus gently by pipetting and add to the cells (+ 6 migrograms/ml of polybrene). Incubate the cells at 37°C overnight.
  • Selection of cells Day 3: change medium and start selection for stably transduced cells
Lentiviral infection of target cells Graph


created over 15 years ago (2 March 2009)    last modified over 12 years ago (28 September 2011)   [ RDF Rdf ]   [ RelFinder Relfinder ]