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Lentiviral infection of target cells
Laboratory protocol
Lentiviral infection of target cells:
Day 1: seed cells
Day 2: remove the medium from the cells. Mix the medium containing the virus gently by pipetting and add to the cells (+ 6 ?g/ml of polybrene). Incubate the cells at 37°C overnight.
Day 3: change medium and start selection for stably transduced cells
Protocol Steps:
-
Cell seeding
Day 1: seed cells
-
Incubation of cells
Day 2: remove the medium from the cells. Mix the medium containing virus gently by pipetting and add to the cells (+ 6 migrograms/ml of polybrene). Incubate the cells at 37°C overnight.
-
Selection of cells
Day 3: change medium and start selection for stably transduced cells
- biomaterial type
- Lentivirus
created over 16 years ago
(2 March 2009)
last modified over 14 years ago
(28 September 2011)
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