has knowledgeable contact person
is related to
Protocols  >  Laboratory protocol  >  Lentiviral infection of tar...

Lentiviral infection of target cells

Laboratory protocol

Lentiviral infection of target cells:
Day 1: seed cells
Day 2: remove the medium from the cells. Mix the medium containing the virus gently by pipetting and add to the cells (+ 6 ?g/ml of polybrene). Incubate the cells at 37°C overnight.
Day 3: change medium and start selection for stably transduced cells

Protocol Steps:
  • Cell seeding Day 1: seed cells
  • Incubation of cells Day 2: remove the medium from the cells. Mix the medium containing virus gently by pipetting and add to the cells (+ 6 migrograms/ml of polybrene). Incubate the cells at 37°C overnight.
  • Selection of cells Day 3: change medium and start selection for stably transduced cells
Lentiviral infection of target cells Graph


created over 8 years ago (2 March 2009)    last modified over 6 years ago (28 September 2011)   [ RDF Rdf ]   [ RelFinder Relfinder ]