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Production of lentiviruses for RNAi and gene expression
Laboratory protocol
Production of lentiviruses:
Producing lentivirus in 293 FT Cells:
Day 1: 4 x 106 293 FT in 10 ml DMEM (+10 % FCS (Tet free), +P/S) in a 10cm plate
Day 2: transfection
Day 3: change of medium
Day 4: harvesting
Protocol Steps:
-
Plating of cells
Day 1: 4 x 10^6 293 FT in 10 ml DMEM (+10 % FCS (Tet-frei), +P/S) in a 10cm plate
-
Transfection
Day 2: transfection
-
Change of medium
Day 3: change of medium
-
Harvesting
Day 4: harvesting (virus-containing)
- biomaterial type
- Lentivirus
created over 15 years ago
(2 March 2009)
last modified over 13 years ago
(28 September 2011)
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