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Protocols  >  Laboratory protocol  >  Production of lentiviruses ...

Production of lentiviruses for RNAi and gene expression

Laboratory protocol

Production of lentiviruses:

Producing lentivirus in 293 FT Cells:
Day 1: 4 x 106 293 FT in 10 ml DMEM (+10 % FCS (Tet free), +P/S) in a 10cm plate
Day 2: transfection
Day 3: change of medium
Day 4: harvesting

Protocol Steps:
Production of lentiviruses for RNAi and gene expression Graph


created over 10 years ago (2 March 2009)    last modified over 7 years ago (28 September 2011)   [ RDF Rdf ]   [ RelFinder Relfinder ]