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FACS staining for activation markers (e.g. CD80)
Laboratory protocol
a) Incubate cells in Fc-block (1:50 in PBS + 2 % FCS) 30 min on ice
b) Staining of the cells in PEalphaDC80 in PBS + 2 % FCS +
c) Fc-block for 30 min on ice
d) FACS analysis
Protocol Steps:
-
Incubation of cells
Incubate cells in Fc-block (1:50 in PBS + 2 % FCS) 30 min on ice
-
Staining
Staining of the cells in PEalphaDC80 in PBS + 2 % FCS
-
Fc-block
Fc-block for 30 min on ice
-
FACS analysis
- biomaterial type
- CD80
created over 15 years ago
(2 March 2009)
last modified over 13 years ago
(28 September 2011)
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