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Improving the efficacy of DC vaccines - ArcelisTM

Laboratory protocol

Argos has developed a novel DC maturation method that dramatically improves DC immunopotency as assessed by in vitro assays compared to DCs matured using the common ‘cytokine cocktail’ method. This new maturation protocol involves transfecting CD40L-encoding RNA along with the tumor RNA payload after maturation with TNF-alpha, IFN-gamma, and PGE2. DC prepared by this method secrete large amounts of IL-12 and no IL-10. This DC platform is now known as ArcelisTM.

Protocol Steps:
  • DC maturation This step of the new maturation protocol involves maturation with TNF-alpha, IFN-gamma, and PGE2.
  • Transfection Transfection of CD40L-encoding RNA along with the tumor RNA payload.
Improving the efficacy of DC vaccines - ArcelisTM Graph


created over 9 years ago (2 March 2009)    last modified over 6 years ago (28 September 2011)   [ RDF Rdf ]   [ RelFinder Relfinder ]