Macrophages and myeloid DC, but not plasmacytoid DC, produce IL-10 in response to MyD88- and TRIF- dependent TLR signals, and TLR-independent signals.

Boonstra, A., Rajsbaum. R., Holman, M., Marques, R., Asselin-Paturel, C., Pereira, J.P., Bates, E.M., Akira, S., Vieira, P., Liu, Y-J., Trinchieri, G., and O’Garra,A
J.Immunol. 177, 7551 – 7558

We have previously reported that mouse plasmacytoid dendritic cells (DC) produce high levels of IL-12p70, whereas bone marrow-derived myeloid DC and splenic DC produce substantially lower levels of this cytokine when activated with the TLR-9 ligand CpG. We now show that in response to CpG stimulation, high levels of IL-10 are secreted by macrophages, intermediate levels by myeloid DC, but no detectable IL-10 is secreted by plasmacytoid DC. MyD88-dependent TLR signals (TLR4, 7, 9 ligation), Toll/IL-1 receptor domain-containing adaptor-dependent TLR signals (TLR3, 4 ligation) as well as non-TLR signals (CD40 ligation) induced macrophages and myeloid DC to produce IL-10 in addition to proinflammatory cytokines. IL-12p70 expression in response to CpG was suppressed by endogenous IL-10 in macrophages, in myeloid DC, and to an even greater extent in splenic CD8alpha(-) and CD8alpha(+) DC. Although plasmacytoid DC did not produce IL-10 upon stimulation, addition of this cytokine exogenously suppressed their production of IL-12, TNF, and IFN-alpha, showing trans but not autocrine regulation of these cytokines by IL-10 in plasmacytoid DC.

URL: http://www.jimmunol.org/cgi/content/full/177/11/7551

Pub Med: http://www.ncbi.nlm.nih.gov/pubmed/17114424

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