has knowledgeable contact person
is document about
Documents and Publications  >  report  >  journal article  >  Direct access to CD4+ T cel...

Direct access to CD4+ T cells specific for defined antigens according to CD154 expression.

journal article

Frentsch M, Arbach O, Kirchhoff D, Moewes B, Worm M, Rothe M, Scheffold A, Thiel A.
Nat Med. 2005 Oct;11(10):1118-24. Epub 2005 Sep 25.

The direct assessment of T helper (T(H))-cell responses specific for antigens is essential to evaluate pathogenic and protective immunity. Presently, analysis and isolation of antigen-specific T(H) cells is restricted to cells that produce cytokines, or can be performed only with a rare selection of specific peptide major histocompatibility complex class II (MHC II) multimers. Here we report a new method that enables the assessment and isolation of T(H) cells specific for a defined antigen according to CD154 expression induced after stimulation in vitro. We show that antigen-induced CD154 expression is highly sensitive and specific for human and mouse antigen-specific T(H) cells. Moreover, the isolation of antigen-specific CD154(+) T(H) cells necessitates only surface staining with antibodies, thereby enabling the fast generation of antigen-specific T(H) cell lines. Our approach allows assessment of T(H) cells with a defined specificity for the combined quantitative and qualitative analysis of T(H)-cell immunity as well as for the isolation of specific T(H) cells for targeted cellular immunotherapies.

URL: http://www.nature.com/nm/journal/v11/n10/abs/nm1292.html

Pub Med: http://www.ncbi.nlm.nih.gov/pubmed/16186818

created over 12 years ago (2 March 2009)    last modified over 10 years ago (28 September 2011)   [ RDF Rdf ]   [ RelFinder Relfinder ]