Gene-silencing in human DC by shRNA approaches

We are currently performing gene silencing experiments in human monocyte-derived DCs (MDDCs) with the dual aim of generating relevant knowledge and data on

(i) the transcriptional regulation of DC differentiation, and
(ii) the safe and rationale in vitro manipulation of gene expression in DCs.

One of the two different approaches that have been set up in parallel is:

Lentiviral transduction of shRNAs: to this aim, shRNA specific for IRF4 and STAT3 are in the process to be cloned into the pLVTHM lentiviral vector (obtained from the repository Addgene). This vector has been already successfully used to generate self-inactivating lentiviral vectors previously tested in DCs for transfection efficiency and absence of effects on their phenotype. A self-inactivating lentiviral vector expressing shRNA specific for STAT3 is now available in the laboratory, and experiments are in progress to optimize their transduction efficiency and silencing in MDDCs.

• molecule type
• small hairpin RNA,
• gene,
• signal transducer and activator of transcription 3,
• interferon regulatory factor 4

• cell type
• Biomaterial

• organism type
• Homo sapiens

• experimental design type
• genetic modification design: knock-out experiment

created over 16 years ago (2 March 2009)    last modified over 13 years ago (28 September 2011)   [ RDF ]   [ RelFinder ]