Evaluation of the role of Src kinases in DCs during synergic engagement of TLR8 with either TLR4 or TLR3

Data has been obtained on the role of Src kinases in DCs during synergic engagement of TLR8 with either TLR4 or TLR3.

Using specific inhibitors we have previously found that src-family tyrosine kinases are required for cytokines and chemokines production during maturation of human monocyte-derived dendritic cells (DCs) upon stimulation with several toll like receptor (TLR) agonists. We have now evaluated the role of Src kinases in DCs during synergic engagement of TLR8 with either TLR4 or TLR3. We tested if src-family tyrosine kinases inhibitors had any effect on the high levels of cytokine production induced in monocyte-derived DCs upon the synergistic stimulation of TLRs. Using the DNA microarray technology we have also analyzed which are the genes that require activation of Src kinases during TLR3 stimulation of monocyte-derived DCs. This pathway analysis will be of help in the elucidation of the molecular mechanism by which Src kinases regulate inflammatory cytokine production in DCs.

• stimulus type
• Toll like receptor 4,
• Toll like receptor 3,
• Toll like receptor 8

• molecule type
• SRC human

• organism type
• Homo sapiens

created over 16 years ago (2 March 2009)    last modified over 13 years ago (28 May 2012)   [ RDF ]   [ RelFinder ]