This tissue was taken from a surgical resection of a melanotic lesion. The electrophoresis product will then be used to generate dendritic cells from the monocytes which in their turn will be used as vaccines.

Patients underwent surgical removal of a metastatic lesion and leukapheresis at the beginning of the trial to provide sources for tumor antigens as well as monocytes and T cells, which will be isolated from the leukapheresis product by elutriation.

PBMC were isolated from this buffy coat. Subsequently, monocytes were isolated from the PBMC and dendritic cells generated from the monocytes.