From the CD14- PBMCs, untouched naive CD4+ T cells were isolated with a combination of magnetic sorting.

These adherent peripheral blood monocytes are produced from leukapheresis from HLA-A1 and/or HLA-A2 positive patients with stage III/IV melanoma, used to generate DCs from. The latter will subsequently be used to vaccinate the HLA-A1 and/or HLA-A2 positive patients.

PBMC were isolated from buffy coat. Subsequently, monocytes were isolated and differentiated into dendritic cells.

PBMC are Leukapheresis derived and are loaded with peptide pools, each consisting of 10 15-mers, which overlap with 11 aminoacids.