clinical grade immature and cytokine cocktail matured monocyte derived human dendritic cells (DC)

A comprehensive protein inventory of clinical grade immature and cytokine cocktail matured (Il-6, IL-1 beta, TNF alpha, PGE2; 48 hours) monocyte derived human dendritic cells (DC) from a healthy donor has been established by using high accuracy, high sensitivity protein identification technology. We have identified 2794 proteins in DCs by liquid chromatography tandem mass spectrometry. Prior to MS analysis, DC were lysed and divided into a soluble fraction containing cytosolic proteins and into an insoluble fraction enriched for membrane containing proteins. Highly complex protein mixtures of DC cellular fractions were separated on 1 D SDS-PAGE into 20 gel slices (fractions), followed by in-gel digestion with trypsin. Purified tryptic peptides were analysed in triplicate by LC-MS/MS using a linear ion trap/ Fourier Transform (LTQ-FT) hybrid mass spectrometer.

• organism type
• Homo sapiens

created over 16 years ago (2 March 2009)    last modified over 13 years ago (28 September 2011)   [ RDF ]   [ RelFinder ]