IL-10 in colitis model

We have investigated the respective role of IL-10 and IDO, both required for Th1 suppression in this model. As IL-10 has been shown to indirectly dampen Th1 responses through the inhibition of IL-12 production by antigen-presenting-cells, we monitored mRNA expression specific for the inducible chain IL-12 p35 in lymph nodes draining the site of injection or in mesenteric lymph nodes of mice instilled intra-colonically with TNBS. Our results clearly show that IL-12 production early after the onset of the response was unaffected by anti-CTLA-4 treatment. By contrast, our data confirm previous data showing that IL-10 is critical for IDO production by DC in vitro, suggesting that IL-10 may act upstream by modulating the expression of IDO. As IDO expression was detected in the colon but not in the spleen or mesenteric lymph nodes, we postulate that IDO may act as anti-inflammatory agent in the intestine and that IL-10 may inhibit Th1 activation in the lymphoid organs and enhance IDO expression in the gut.

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