We have experience on performing immunofluorescence assays. As an example, an assay on ordered sequential sections was used to analyze the infiltration and destruction of solid tumors by CTLs.

There is know-how on classical MLR assays in our lab. They are used, for instance, for characterization of silenced cells.

In our laboratory, we have experience in performing CBA assays. We use them, e.g., in the monitoring of vaccine induced T cell responses.

Our lab has know-how in performing 3H-thymidine-incorporation assays. This assay was used in the monitoring of vaccine induced T cell responses.

In our laboratory we perform 51Cr-release assays, for instance in the monitoring of vaccine induced T cell responses.

Our lab has expertise in performing thymidine-incorporation assays. The assay is used, for instance, for the monitoring of the vaccine induced specific anti-tumor T cell responses.

We have know-how about LDA assays; for instance, we use LDA assays to monitor vaccine induced T cell responses.

In our laboratory we carry out multiplex assays. For instance, multiplex analysis was used to study the response of human MoDC to yeast, spheroplasts, pseudohyphae and spores.

In our lab, we have know-how about performing MPLC assays, such as using it in the establishment of immunomonitoring vaccination trials with RNA transfected DC by using overlapping peptides.

We have expertise in performing toll-like receptor (TLR)-driven luciferase reporter assays. For example, in a study on the activation of DC we applied a TLR-driven luciferase reporter assay which showed dose-dependent activation of TLR2, TLR3, and TLR8, which was independent of the pseudotype, production, or transduction protocol and was abrogated on heat inactivation.

Our lab has experience in carrying out ELISPOT analyses. For instance, an IFNgamma ELISPOT assay with PBMC loaded with peptide pools (each consisting of 10 15-mers, which overlap with 11 aminoacids) was done for a study on antigen recognition.