This platform with its enhanced GMP facilities for production of clinical-grade DC is established in Erlangen DE (P6, Schuler / Schuler-Thurner). However, later it was decided to seek a licence for the production of GMP-grade mRNA that will be used for the (revised) multi-centre clinical trial.

A Zeiss LSM 510 confocal microscope is available for use in the lab.

In march 2009 we obtained the new BD ARIA II, which not only allows 14 parameter flow cytometry, but also sorting of even very small antigen specific T cell subpopulations for further molecular analysis in close collaboration with Pierre Coulie´s group in Brussels. [PhD student Steve Voland]

We have a confocal microscope available. This microscope was is used, for instance, for the characterization of antigen depots.

An Illumina transcriptional profiling platform is available for microarray analyses.

A GenePix 4000 microarray scanner is available in our lab (manufacturer: Axon, Foster City, CA).

The yeast oligonucleotide array was constructed using the S. cerevisiae Genome Oligo Set ™ (Operon Technologies, CA, USA) composed of 6240 optimized oligonucleotides (70mers) each representing one yeast gene.

The FAX analyser available in the laboratory is used, e.g., to measure transfection efficiency.

There is a MACS separator (Miltenyi Biotech) at the disposal of our lab. It is used, for example, to purify CD8 T cells.

In the lab, there is a multi-photon microscope available. It is used, for instance, in the analysis of in vivo distribution and motility of NK cells.

The flow cytometer at the disposal of the laboratory is used, for example, to assess Th subsets.

The flow cytofluorometer available to the laboratory is used, for example, for the assessment of differentiation, maturation, and intracellular cytokine production of DCs.

The FACS analyser available to the laboratory is used, for instance, to analyse stained cells.

This spectrometer was, among other things, used for an analysis of the integral plasma membrane proteome of D1 cells.

This flow cytometer was used eg., for an analysis of CD11c populations in the lung and spleen during acute aerosol MTb infection.

The integral plasma membrane proteome of D1 cells stimulated for 24 hours with LPS has been analyzed with this LC-MS/MS analyser and compared to the results obtained from immature D1 cells.

The Challenge of Cancer: Individual Cancers and Mutations Every individual´s cancer is unique. Therefore, the most effective treatment is one customized to a particular cancer´s genetic configuration. Genetic mutations and chromosomal abnormalities are commonly associated with human cancers. Unfortunately, since the genetic mutations leading to the development of cancer are often random events, every patient's tumor can contain a unique repertoire of antigens. This characteristic of human cancer requires that each patient's immune system be able to recognize and ...

Our laboratory has a mass spectrometer at its disposal.

A Luminex flow cytometer is available to our lab. It is used, among other things, to quantify the TNFalpha and IFNgamma secretion in a study on antigen recognition.

There is a two-photon intravital microscope available in the laboratory. It was used, among other things, to analyze the infiltration and destruction of solid tumors by CTLs.