MASCOT probability-based scoring

Iterative calibration algorithms were used to achieve a final, absolute mass accuracy of better than 10 parts per million (p.p.m.) for all peptide ions.

These high-accuracy spectra were searched against a human protein database, using MASCOT probability-based scoring in which the fragment ions are matched against the calculated fragments of all tryptic peptides from the human sequences. Additional constraints were set by searches against a reversed human database to minimize false positive protein identifications.

created over 15 years ago (30 March 2009)    last modified over 13 years ago (28 September 2011)   [ RDF ]   [ RelFinder ]