Transient immunoreceptor expression by RNA transfection

The method is about transfecting CD4(+) and CD8(+) T cells with mRNA encoding recombinant immunoreceptors for use in the adoptive immunotherapy of cancer.
CD4(+) and CD8(+) T cells were efficiently transfected with immunoreceptors specific for ErbB2 and CEA. The immunoreceptor expression was transient with half-maximal expression at day 2 and no detectable immunoreceptor expression at day 9 after electroporation.

Opposed to standard procedures using retroviral gene transduction to constitutively express immunoreceptors in T cells, transient immunoreceptor expression by RNA transfection should should minimize the risk of persistence of potential autoaggression.

created over 14 years ago (22 February 2010)    last modified over 13 years ago (28 September 2011)   [ RDF ]   [ RelFinder ]