Incubation at 65 °C for 12–15 h.

The next day the peptides and KLH are replaced and incubated for further 4h and then used for quality controls and intradermal administration.

After 10’ of incubation in 65 °C water bath, the samples are incubated on ice.

Incubate cells in Fc-block (1:50 in PBS + 2 % FCS) 30 min on ice

Day 2: remove the medium from the cells. Mix the medium containing virus gently by pipetting and add to the cells (+ 6 migrograms/ml of polybrene). Incubate the cells at 37°C overnight.