Elutriation of the leukapheresis product.

After the centrifugation (10’ at 5000 rpm) the supernatant is transferred into a pre-spun (5’ at 1500 rpm) 50 ml Phase Lock Gel tube (Eppendorf 0032 005.330) and 4 ml of chloroform (Fischer BP1145-1) are added into the falcon.

Day 4: harvesting (virus-containing)

Day 3: change medium and start selection for stably transduced cells