refolded CD1 molecules are used to generate CD1 tetramers

The DC vaccine will consist of monocyte derived DC loaded with tumor lysate generated from the excised lesion.

Generation of tumor lysate loaded DC.

DCs will be generated from monocytes.

DCs are generated from adherent peripheral blood monocytes.

The yeast oligonucleotide array is constructed using the S. cerevisiae Genome Oligo Set ™ (Operon Technologies, CA, USA) composed of 6240 optimized oligonucleotides (70mers) each representing one yeast gene.