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Protocol Steps  >  centrifugation step  >  Centrifugation

Centrifugation

centrifugation step

Protocol: Total RNA Isolation from S. cerevisiae

After the spin (10’ at 3,000 rpm), the supernatants are precipitated in RNase-free centrifuge tube by adding 1/10 volume 3 M NaAcetate (pH 5.3) and 2.5 volumes of cold ETOH 100% overnight at -20°C. Centrifugation (30’ at 12000 rpm).

created over 14 years ago (2 March 2009)    last modified over 11 years ago (28 September 2011)   [ RDF Rdf ]   [ RelFinder Relfinder ]