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Protocol Steps  >  substance detection step  >  Screening of the microcultures

Screening of the microcultures

substance detection step

Protocol: Detection, counting, and eventually cloning CD4 or CD8 T cells against any peptide encoded by a given gene and presented by any HLA molecule

All the individual microcultures are then screened for recognition of autologous EBV-B cells transduced with a retrovirus encoding the protein. Non transduced EBV-B cells are used as control stimulator cells.

created over 13 years ago (4 January 2010)    last modified over 11 years ago (28 September 2011)   [ RDF Rdf ]   [ RelFinder Relfinder ]