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Protocol Steps  >  stimulation step  >  Stimulation of PBMC

Stimulation of PBMC

stimulation step

Protocol: Detection, counting, and eventually cloning CD4 or CD8 T cells against any peptide encoded by a given gene and presented by any HLA molecule

PBMC are stimulated in limiting dilution conditions with a pool of overlapping peptides (15 amino acids) covering the protein.

created over 13 years ago (4 January 2010)    last modified over 11 years ago (28 September 2011)   [ RDF Rdf ]   [ RelFinder Relfinder ]