Real Time PCR
Standard operating procedureA retrotranscriptional reaction is performed on RNA extracted from T cells or DCs. Transcripts for interested genes are quantified by real-time quantitative PCR on an Perkin-Elmer Applied Biosystems with predesigned TaqMan Gene Expression Assays according to the manufacturer’s instructions.
Protocol Steps:

- biomaterial type
- ribonucleic acids,
- T cell,
- dendritic cell,
- bone
created over 16 years ago
(2 March 2009)
last modified over 14 years ago
(28 September 2011)
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