Real Time PCR
Standard operating procedureA retrotranscriptional reaction is performed on RNA extracted from T cells or DCs. Transcripts for interested genes are quantified by real-time quantitative PCR on an Perkin-Elmer Applied Biosystems with predesigned TaqMan Gene Expression Assays according to the manufacturer’s instructions.
Protocol Steps:
- biomaterial type
- ribonucleic acids,
- T cell,
- dendritic cell,
- bone
created over 16 years ago (2 March 2009) last modified over 13 years ago (28 September 2011)  [ RDF ]  [ RelFinder ]