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Protocols  >  Standard operating procedure  >  Real Time PCR

Real Time PCR

Standard operating procedure

A retrotranscriptional reaction is performed on RNA extracted from T cells or DCs. Transcripts for interested genes are quantified by real-time quantitative PCR on an Perkin-Elmer Applied Biosystems with predesigned TaqMan Gene Expression Assays according to the manufacturer’s instructions.

Protocol Steps:
Real Time PCR Graph

created over 14 years ago (2 March 2009)    last modified over 11 years ago (28 September 2011)   [ RDF Rdf ]   [ RelFinder Relfinder ]