Priming of naive T cells
Laboratory protocolUntouched naive CD4+ T cells are co-coltured with fungi -pulsed DCs and then the polarization is assessed by flow cytofluorimetry and cytokine production.
Protocol Steps:
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Coculture
Untouched naive CD4+ T cells are co-coltured with fungi -pulsed DCs
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Assessment of polarization
The polarization is assessed by flow cytofluorimetry and cytokine production.
created over 16 years ago (2 March 2009) last modified over 13 years ago (28 September 2011)  [ RDF ]  [ RelFinder ]