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In vitro CD8 T cell stimulatory capacity of DC

Laboratory protocol

Purified CD8 T cells using MACS cell separation were co-cultured with electroporated DC. After several stimulations, the CD8 T cells become evaluated on their cytokine secretion, cytotoxicity and % of antigen specific T cells.

Protocol Steps:
  • MACS cell purification CD8 T cells are purified using MACS cell separation.
  • electroporation Dendritic cells are electroporated.
  • Coculture Purified CD8 T cells using MACS cell separation are co-cultured with electroporated DC.
  • Stimulation of cells This step is repeated several times to do several stimulations.
  • Evaluation The CD8 T cells become evaluated on their cytokine secretion, cytotoxicity and % of antigen specific T cells.
In vitro CD8 T cell stimulatory capacity of DC Graph


created over 9 years ago (2 March 2009)    last modified over 6 years ago (28 September 2011)   [ RDF Rdf ]   [ RelFinder Relfinder ]