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In vitro CD8 T cell stimulatory capacity of DC
Laboratory protocolPurified CD8 T cells using MACS cell separation were co-cultured with electroporated DC. After several stimulations, the CD8 T cells become evaluated on their cytokine secretion, cytotoxicity and % of antigen specific T cells.
Protocol Steps:
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MACS cell purification
CD8 T cells are purified using MACS cell separation.
-
electroporation
Dendritic cells are electroporated.
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Coculture
Purified CD8 T cells using MACS cell separation are co-cultured with electroporated DC.
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Stimulation of cells
This step is repeated several times to do several stimulations.
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Evaluation
The CD8 T cells become evaluated on their cytokine secretion, cytotoxicity and % of antigen specific T cells.
- biomaterial type
- T cell,
- dendritic cell,
- CD8
created over 16 years ago
(2 March 2009)
last modified over 13 years ago
(28 September 2011)
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