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Single step procedure for the in vitro maturation and antigen loading of human moDC

Laboratory protocol

A single step procedure for the in vitro maturation and antigen loading of human moDC was optimized. DC were electroporated with mRNA encoding tumor antigens and a putative TLR3 ligand. In vitro stimulation of naïve T cells indicated the superiority of this approach compared to in vitro stimulation with DC matured with inflammatory cytokines followed by electroporation with TAA encoding mRNA.

Protocol Steps:
Single step procedure for the in vitro maturation and antigen loading of human moDC Graph


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