In vitro charging of moDC with antigens
Laboratory protocol
We previously described mRNA electroporation as an efficient gene delivery method to introduce tumor-antigens (Ag) into murine immature dendritic cells (DC).
We have further optimized the protocol and evaluated the capacity of mRNA-electroporated DC as a vaccine for immunotherapy.
Protocol Steps:

- biomaterial type
- immature dendritic cell,
- Biomaterial,
- messenger RNA,
- Mus musculus
created over 16 years ago
(2 March 2009)
last modified over 13 years ago
(28 September 2011)
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