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Protocols  >  Laboratory protocol  >  In vitro charging of moDC w...

In vitro charging of moDC with antigens

Laboratory protocol

We previously described mRNA electroporation as an efficient gene delivery method to introduce tumor-antigens (Ag) into murine immature dendritic cells (DC).

We have further optimized the protocol and evaluated the capacity of mRNA-electroporated DC as a vaccine for immunotherapy.

Protocol Steps:
In vitro charging of moDC with antigens Graph


created over 9 years ago (2 March 2009)    last modified over 6 years ago (28 September 2011)   [ RDF Rdf ]   [ RelFinder Relfinder ]