Transfection of DC with RNA encoding E/L-Selectin
Laboratory protocol
We have efficiently transfected DC with RNA encoding a functional protein (E/L-Selectin) which allows entry of DC into LN from HEV.
RNA transfected human DC could be frozen and thawed without loosing their functionality.
Protocol Steps:
- biomaterial type
- ribonucleic acids,
- dendritic cell,
- high endothelial cells,
- human lymph node,
- selectin
created over 16 years ago (2 March 2009) last modified over 13 years ago (28 September 2011)  [ RDF ]  [ RelFinder ]