Optimization for the mass spectrometry analysis methods to be used in the generation of a initial proteomic map of mouse DCsOther protocol
The optimization for the mass spectrometry analysis methods to be used in the generation of a initial proteomic map of mouse DCs (WP2) and stimuli-related phosphorylation cascades (WP5) was carried out by Lyris M. F. de Godoy (Post-doc) and Jesper V. Olsen (PhD student). Recently, our lab has been developing new techniques in quantitative proteomics and has also acquired new instrumentation to assess these types of questions in depth. This, of course, involves a lot of optimization and, consequently, large amounts of sample. However, to obtain high numbers of dendritic cells is not a trivial process, and can also be very expensive. For that reason, we have been using other model systems, like yeast, for all the general optimization steps, to have a basic set-up before we perform the experiments with the DCs.