Recommendation of assays for trials with peptide loaded DC (> 60 pat.) routine immunomonitoring at frequent intervalsPost-clinical protocol
In ongoing trials with peptide loaded DC (> 60 pat.) routine immunomonitoring at frequent intervals showed a high correlation between different assays (IFN gamma ELISPOT; modified MLPC = combination of limiting dilution, multiple peptide restimulation, tetramer detection).
Both assays demonstrated declining responses upon extension of vaccination intervals. We suggest:
IFN gamma ELISPOT as basic assay (costs, workload) that can be frequently performed in every patient for routine monitoring
Modified MLPC (combination of limiting dilution, multiple peptide restimulation, Tetramer detection) allowing true assessment of precursor frequencies, may only be performed at leukapheresis time points in selected patients due to extensive requisitions (blood volume, costs, workload).