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Immunization with mannosylated peptide induces poor T cell effector functions despite enhanced antigen presentation.

journal article

Kel JM, de Geus ED, van Stipdonk MJ, Drijfhout JW, Koning F, Nagelkerken L.
Int Immunol. 2008 Jan;20(1):117-27. Epub 2007 Nov 15.

In this study, we investigated the development of T cell responses in mice after administration of a mannosylated ovalbumin peptide (M-OVA(323-339)). Immunization with M-OVA(323-339) in complete adjuvant resulted in enhanced antigen presentation in draining lymph nodes. Monitoring the fate of CFSE-labeled ovalbumin peptide-specific TCR transgenic CD4(+) T cells revealed that immunization with M-OVA(323-339) induced normal clonal expansion, recirculation and CD62L expression of antigen-specific T cells in vivo. However, these T cells developed only poor effector functions, reflected by minimal IFN-gamma production, low IgG2a levels in serum and poor peptide-specific delayed-type hypersensitivity (DTH) responses. This diminished inflammatory response was associated with decreased infiltration of T cell blasts and macrophages. Importantly, also mice with functional effector T cells did not mount a robust DTH response after a challenge with M-OVA(323-339) in the ear, although their T cells responded normally to M-OVA(323-339) in vitro. In conclusion, mannosylated peptide induces proliferation of T cells with impaired T(h)1 cell effector functions and additionally abrogates the activity of pre-existing effector T cells.

URL: http://intimm.oxfordjournals.org/cgi/content/full/20/1/117

Pub Med: http://www.ncbi.nlm.nih.gov/pubmed/18024466

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