Ligand-conjugated quantum dots monitor antigen uptake and processing by dendritic cells.

Cambi A, Lidke DS, Arndt-Jovin DJ, Figdor CG, Jovin TM
Nano Lett. 2007 Apr; 7(4):970-7.

The dendritic cell (DC) specific pathogen-uptake receptor (DC-SIGN) internalizes antigens for degradation and presentation onto MHC molecules. At the cell membrane, DC-SIGN forms nanoclusters that facilitate virus capture. However, internalized viruses, such as HIV-1, escape degradation. Here, we exploit ligand-conjugated, virus-sized, highly photostable quantum dots (QDs) to monitor in living cells antigen binding, entry, and trafficking. The antigen-coated QDs specific uptake and persistence in live DCs open the possibility for tracking antigen-presenting cells in vivo.

URL: http://pubs.acs.org/cgi-bin/abstract.cgi/nalefd/2007/7/i04/abs/nl0700503.html

Pub Med: http://www.ncbi.nlm.nih.gov/pubmed/17388641

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