Read-out system for antigen presentation
Using MAGE-A3 as a model antigen we established a functional read-out system by transfection of CD4 T cells with RNA encoding T cell receptors recognizing MHC DP4 restricted MAGE-A3 epitopes.
Antigen-loading strategies for DC: comparison of lipofection of tumor-antigen-encoding RNA and RNA electroporation into DC; RNA electroporation with loading of DC with anti-DEC205-antigen constructs