Overlapping peptide library technique for monitoring RNA-DC vaccinated patients
Using this peptide library technique and ELISPOT analysis patients´ PBMC are pre-screened for CD4- and CD8- T cell responses and individual sets of 4 to 8 peptides chosen for further analysis.
Tools and Methods
method
Gerold Schuler
Establishment of immunomonitoring vaccination trials with RNA transfected DC by using overlapping peptides covering the whole protein sequence of the tumor antigens / RNAs used
Alexander Steinkasserer