Ex-vivo analysis of peptide-specific T cell functions
comment: Data were obtained from an ex-vivo analysis of different peptide specific T cell functions like degranulation and production of cytokines such as IFN? and TNF? by 8-color flow cytometry. In general, pre-existing immune-responses were rather mono-functional with dominating “IFN?-only”, “TNF?-only” and “degranulation-only” cells in the CD8+ compartment. Vaccination not only increased some of these monofunctional subpopulations but also induced occurrence of tumorantigen specific polyfunctional subsets. The further course of our melanoma patients will show whether these vaccination induced new T-cell “flavours” as well as different patterns of homing receptors do translate into better clinical efficacy.
type: data set
type: preclinical study dataset
related to instance: DERMA-ER-DC 06 trial: RNA-based DC vaccine
uses tool: Multicolor flow cytometer
has organism type: Homo sapiens
has cell type: T cell
has molecule type: interferon
has molecule type: Tumor necrosis factor
has molecule type: CD8
has knowledgeable: Gerold Schuler