Evaluation of different stimulation conditions for the cytometric assessment of antigen-specific Th-cells
Data is available from an evaluation of different stimulation conditions for the cytometric assessment of antigen-specific Th-cells based on CD154 expression induced during short-term in vitro activation, a method established by us during previous DC-THERA activities. This is important and essential for implementation of the technology into clinical immune monitoring in e.g. vaccination trials.
Moreover, the affect of stimulation length was determined. Differences with respect to the induced frequencies of antigen-specific, activated CD154+ Th-cells were minor. Interestingly, longer stimulation times (16h) were characterised by a slightly reduced coefficient of variation. However, longer stimulation times resulted also in slightly enhanced background, implying that overnight stimulations cannot be performed when low frequencies of antigen-specific Th-cells are expected (Meier, Stark et al., 2008).
data set
imaging dataset
preclinical study dataset
CD4-positive T helper cell
Flow cytometric assessment of Th subsets
The influence of different stimulation conditions on the assessment of antigen-induced CD154 expression on CD4+ T cells.
SOPs for the cytometric assessment of Th-cell immunity based on antigen-induced CD154 expression
Alexander Scheffold