NOX2-deficient DCs
We have demonstrated that the NADPH oxidase NOX2 is recruited to DC early phagosomes mediating a sustained production of low levels of reactive oxygen species and causing a maintained alkalynization of the phagosomal lumen. DCs lacking NOX2 show increased antigen degradation due to an enhanced phagosome acidification. As a result, the efficiency of in vitro and in vivo antigen cross presentation is significantly reduced in NOX2-deficient DCs.
Biomaterial
dendritic cell
Sebastian Amigorena
Ariel Savina
Studies of DC phagosomes and cross-presentation using K/O and mutant mouse strains