Activated DC
These dendritic cells were generated from monocytes isolated from buffy coats, activation was induced by lipopolisaccaride (LPS 1microgram/ml, Sigma, St. Louis, MO), by Curdlan (100 micrograms/ml, Wako), by R848 and yeast RNA and by yeast cells in different conditions of culture.
Biomaterial
dendritic cell
Lisa Rizzetto
monocyte
IL-12p70 inhibition by cytochalasin D
DC stimulation with spores or yeasts
DC activation
Phagocytosis assay