siRNA Smart Pool targeted against the STAT3 transcription factor
comment: For transient transfection of synthetic siRNA, commercially available siRNA Smart Pool from Dharmacon targeted against the STAT3 transcription factor have been successfully transfected in human MDDCs (Lipofectamine 2000 as transfection reagent). The efficiency of silencing, as detected by western blot or FACS analysis of the protein was of 50-80% (depending on the donor), and peaked at 72 h post-transfection. Transfection efficiency was reproducibly higher than 80% and did not induce any type I IFN production.
type: Biomaterial
type: small interfering RNA
has knowledgeable: Sandra Gessani
is bio mat used in: Gene-silencing in human DC by siRNA approaches